Mat Test For Leptospirosis

Detection of leptospiral nucleic acid from a clinical specimen.

Mat test for leptospirosis.

The mat or microscopic agglutination test detects the presence of antibodies against leptospira in a dog s blood. 1965 and modified by cole et al. It uses panels of live leptospires ideally recent isolates representing the circulating serovars from the area where the patient became infected. The mat is an excellent test but it also has limitations.

Microscopic agglutination test mat for the diagnosis of leptospirosis and serotyping of leptospires. If the level of antibodies called a titer is high enough or can be shown to be rising over time then infection is confirmed. Mat is considered the gold standard test for diagnosis of leptospirosis. Multilocus sequence typing mlst will be performed on clinical samples that have tested pcr positive.

The microscopic agglutination test mat is the basis of serological diagnosis and classification. 1973 is the most widely used serological test for leptospirosis thiermann and garrett 1983. The mat is best used as a screening test when investigating the possibility of l. What is the mat test for leptospirosis.

Microscopic agglutination test mat mat is regarded as a gold standard method for the diagnosis of leptospirosis but it may be only available in reference laboratories. The microscopic agglutination test mat is the reference test method for the serodiagnosis of leptospirosis both in humans and in animals world health organization who 2003. Isolation of leptospires from a clinical specimen. Laboratory test for diagnosis.

It has unsurpassed specificity but its sensitivity is low compared to elisa igm slide agglutination test sat. World organisation for animal health oie 2008. A 4 fold rise in mat titer between acute and convalescent sera. The majority of leptospirosis cases are identified through mat serology.

Korver department of biomedical research royal tropical institute meibergdreef 39 11005 az amsterdam the netherlands get pdf file. Mat has high sensitivity and detects serovar specific antibodies. Seroconversion with a 4 fold or larger rise in titer between acute and convalescent phase serum specimens obtained 2 weeks or more apart confirms the diagnosis. The centers for disease control and prevention cdc laboratory in atlanta georgia performs the mat using 23 leptospire antigens.

A single elevated mat titer in a patient with a compatible febrile illness and suspected exposure. Hardjo infection in groups or herds of cattle. Laboratory definitive evidence for a confirmed case requires at least one of the following. Does the mat always work.